Peakwise expression - C.albicans AS5_13

30 October 2019

Sample similarity

The following plots attempt to summarize the similarity/differences in expression patterns between samples, based on the glog2-transformed normalized read counts. Samples from the same experimental group should cluster together.

limma's Multi-Dimensional Scaling plot of sample similarity

varistran's biplot, similar to plotMDS but shows genes on the same plot

Split Network visualization of sample similarity.
(Visualization of euclidean distances as a split network. Note: This is not a phylogenetic network.)

Poly(A) tail length distribution

This plot shows the distribution of lengths of poly(A) tail sequence in top expressed features. Its main purpose is to assess data quality. If the plot has many bright spots there may be many identical reads, possibly due to non-random digestion.

Only reads with a poly(A) sequence of four or more bases are used.

AS5_13-Oct-2019-peakwise--pooled-heatmap.png (915.1kb) ⋅ [spreadsheet]