Boag et al., Figure 3 RNAi

14 January 2015

Genewise expression level

Spreadsheet, voom plot

207 with fdr<=0.01

745 with fdr<=0.05



Raw data format: Gld2-pcb19-rep1; Gld2-pcb19-rep2; Gld2-PARN1-rep1; Gld2-PARN1-rep2; Gld2-CAF1-rep1; Gld2-CAF1-rep2; Gld2-PANL2-rep1; Gld2-PANL2-rep2; Gld2-CCF1-rep1; Gld2-CCF1-rep2; Gld2-CCR4-rep1; Gld2-CCR4-rep2; 1-2-cell-egg-rep1; 1-2-cell-egg-rep2
7701 of 31753 features kept after filtering
(required at least one sample with 50 reads)
prior df = 5.4869
s.d. = 0.912299 / sqrt(weight)
noise combined p-value = 1

Genewise tail length

Spreadsheet

185 with fdr<=0.01

765 with fdr<=0.05



Raw data format: Gld2-pcb19-rep1; Gld2-pcb19-rep2; Gld2-PARN1-rep1; Gld2-PARN1-rep2; Gld2-CAF1-rep1; Gld2-CAF1-rep2; Gld2-PANL2-rep1; Gld2-PANL2-rep2; Gld2-CCF1-rep1; Gld2-CCF1-rep2; Gld2-CCR4-rep1; Gld2-CCR4-rep2; 1-2-cell-egg-rep1; 1-2-cell-egg-rep2
3850 of 31753 features kept after filtering
(required sufficient samples with 50 poly(A) reads to fit linear model)
prior df = 13.0033
variance = 27.3^2 / polya_read_count + 0.061535^2 * tail_length^2
noise combined p-value = 0.00633344